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immunohistochemical staining  (Proteintech)


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    Proteintech immunohistochemical staining
    Immunohistochemical Staining, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 255 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunohistochemical+staining/Cd31+Polyclonal+antibody/pmc12963993-174-0-7
    Average 96 stars, based on 255 article reviews
    immunohistochemical staining - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    other:

    Article Title: Mitochondrial DNA drives NLRP3-IL-1β axis activation in microglia by binding to NLRP3, leading to neurodegeneration in Parkinson's disease models.
    Article Snippet: Subsequently, the sections were deparaffinized, hydrated, and incubated with the antibody against TH (1:5000, #25859-1-AP, Proteintech) for immunohistochemical staining using an immunohistochemistry kit (Zhongshan Golden Bridge).

    Article Title: ITGA5 induces mesenchymal transformation to promote gliomas progression via PI3K/AKT/mTORC1 signaling pathway.
    Article Snippet: Western blot, immunohistochemical staining of antibodies included ITGA5 Rabbit mAB (No.27224-1-AP; Proteintech, Wuhan, China; WB 1:2000; IHC 1:100). p-mTOR Rabbit mAB (Code: T56571F; Abmart, Shanghai, China; WB 1:2000). mTOR Rabbit mAB (Code: T55306F; Abmart, Shanghai, China; WB 1:2000).

    Article Title: Integrative analysis of single-cell RNA sequencing, bulk RNA sequencing, and proteomic data identified NOTCH3 as a hub gene contributing to human intervertebral disc fibrosis.
    Article Snippet: Immunohistochemical Analysis Human NP tissues from discs at different degeneration grades were fixed, paraffin-embedded, and sectioned for immunohistochemical staining using an anti-NOTCH3 antibody (Proteintech, 55114-1-AP).

    Article Title: Biodegradable Mg 2+ -releasing piezoelectric scaffold for segmental bone defect repair.
    Article Snippet: Immunohistochemical staining was carried out for COL-I (Proteintech, 28083-1-AP, USA).

    Article Title: MicroRNA-124-3p suppresses lung cancer by targeting ITGB1/PI3K/p-AKT signal transduction pathway.
    Article Snippet: Worldwide, lung cancer is a leading cause of cancer-related death, and non-small cell lung cancer (NSCLC) represents the most common histological subtype.. Numerous studies have demonstrated that microRNAs (miRNAs) play critical roles in NSCLC pathogenesis. microRNA-124–3p (miR-124–3p) has been identified as a tumor-associated miRNA, and we confirmed its downregulation in NSCLC cells.. Functional assays showed that overexpression of miR-124–3p suppresses proliferation and migration of NSCLC cells, whereas its knockdown promotes these malignant phenotypes.

    Article Title: Biodegradable Mg 2+ -releasing piezoelectric scaffold for segmental bone defect repair
    Article Snippet: Immunohistochemical staining was carried out for COL-I (Proteintech, 28083-1-AP, USA).

    Article Title: Integrative analysis of single-cell RNA sequencing, bulk RNA sequencing, and proteomic data identified NOTCH3 as a hub gene contributing to human intervertebral disc fibrosis
    Article Snippet: Human NP tissues from discs at different degeneration grades were fixed, paraffin-embedded, and sectioned for immunohistochemical staining using an anti-NOTCH3 antibody (Proteintech, 55114-1-AP).

    Article Title: Mitochondrial DNA drives NLRP3-IL-1β axis activation in microglia by binding to NLRP3, leading to neurodegeneration in Parkinson’s disease models
    Article Snippet: Subsequently, the sections were deparaffinized, hydrated, and incubated with the antibody against TH (1:5000, #25859-1-AP, Proteintech) for immunohistochemical staining using an immunohistochemistry kit (Zhongshan Golden Bridge).



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    Image Search Results


    Evaluation of mucosal status after tracheal mucosal injury in rabbits . (a) Gross morphology and Masson staining of mucosal defects (n = 3). (b – f) Tracheal samples were collected on Day 10, and RNA sequencing was performed to assess biological differences between Native and Model groups (n = 3). (b) Principal components analysis of samples. (c) Volcano plot of DEGs. (d) KEGG enrichment analysis of DEGs. (e) Chord plot of enriched KEGG terms. (f) Heatmaps of DEGs associated with inflammation and oxidative stress. (g) IF staining of FISH (marker of bacteria, pink), iNOS (marker of M1 macrophages, orange), CD206 (marker of M2 macrophages, green), and immunohistochemical staining of CD31 (marker of endothelial cells) in various samples, blood vessels are denoted by triangles.

    Journal: Bioactive Materials

    Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury

    doi: 10.1016/j.bioactmat.2026.01.026

    Figure Lengend Snippet: Evaluation of mucosal status after tracheal mucosal injury in rabbits . (a) Gross morphology and Masson staining of mucosal defects (n = 3). (b – f) Tracheal samples were collected on Day 10, and RNA sequencing was performed to assess biological differences between Native and Model groups (n = 3). (b) Principal components analysis of samples. (c) Volcano plot of DEGs. (d) KEGG enrichment analysis of DEGs. (e) Chord plot of enriched KEGG terms. (f) Heatmaps of DEGs associated with inflammation and oxidative stress. (g) IF staining of FISH (marker of bacteria, pink), iNOS (marker of M1 macrophages, orange), CD206 (marker of M2 macrophages, green), and immunohistochemical staining of CD31 (marker of endothelial cells) in various samples, blood vessels are denoted by triangles.

    Article Snippet: Immunofluorescence staining of CK14 (Abcam, ab181595), AC-Tub (Proteintech, 66200-1-Ig), ZO-1 (Proteintech, 21773-1-AP), and Immunohistochemical (IHC) staining for CD31 (Servicebio, S1002) were conducted to reveal the conditions of mucosal regeneration, according to previous literature [ ].

    Techniques: Staining, RNA Sequencing, Marker, Bacteria, Immunohistochemical staining

    Gel-AgNA/MgGA MN remodel the microenvironment after TMI by regulating infection, inflammation, oxidative stress, and vascular disruption. (a) FISH (marker of bacteria, red) staining of harvested samples after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b, c) Quantitative analysis of bacterial coverage and fluorescence intensity of FISH (n = 9). (d) Heatmaps of DEGs related to inflammation and oxidative stress. (e) RT-PCR analysis showing the relative expression levels of IL-1, TNF-α, HIF-1α, and IL-6 (n = 3). (f) IF staining of iNOS (marker of M1 macrophages, red) and CD206 (marker of M2 macrophages, green). (g) Quantitative analysis of iNOS and CD206 fluorescence intensity. (h) RT-PCR analysis showing the relative expression levels of Arg1 and CD206. (i) IF staining of CD31 (marker of blood vessels, red). (j, k) RT-PCR analysis showing the relative expression levels of VEGF and HIF-1α (n = 3).

    Journal: Bioactive Materials

    Article Title: Spatiotemporally engineered microneedle for microenvironment remodeling propels mucosal regeneration after tracheal mucosal injury

    doi: 10.1016/j.bioactmat.2026.01.026

    Figure Lengend Snippet: Gel-AgNA/MgGA MN remodel the microenvironment after TMI by regulating infection, inflammation, oxidative stress, and vascular disruption. (a) FISH (marker of bacteria, red) staining of harvested samples after treated with Gel, Gel-AgNA, Gel-MgGA, and Gel-AgNA/MgGA MN. (b, c) Quantitative analysis of bacterial coverage and fluorescence intensity of FISH (n = 9). (d) Heatmaps of DEGs related to inflammation and oxidative stress. (e) RT-PCR analysis showing the relative expression levels of IL-1, TNF-α, HIF-1α, and IL-6 (n = 3). (f) IF staining of iNOS (marker of M1 macrophages, red) and CD206 (marker of M2 macrophages, green). (g) Quantitative analysis of iNOS and CD206 fluorescence intensity. (h) RT-PCR analysis showing the relative expression levels of Arg1 and CD206. (i) IF staining of CD31 (marker of blood vessels, red). (j, k) RT-PCR analysis showing the relative expression levels of VEGF and HIF-1α (n = 3).

    Article Snippet: Immunofluorescence staining of CK14 (Abcam, ab181595), AC-Tub (Proteintech, 66200-1-Ig), ZO-1 (Proteintech, 21773-1-AP), and Immunohistochemical (IHC) staining for CD31 (Servicebio, S1002) were conducted to reveal the conditions of mucosal regeneration, according to previous literature [ ].

    Techniques: Infection, Disruption, Marker, Bacteria, Staining, Fluorescence, Reverse Transcription Polymerase Chain Reaction, Expressing